Application

The antibody may be used in various immunochemical techniques including Immunoblot1-6(~104 kDa), Immunofluorescence1,6, Immunoprecipitation,3 Electron Microscopy7 and Immunoaffinity purification of the Golgi adaptor complex AP-15.

Biochem/physiol Actions

AP2 is responsible for Clathrin-mediated trafficking from the plasma membrane to the endosomes, while AP1 mediates trafficking from the Golgi to endosomes and from the endosomal system to other destinations.10 Biochemical analysis of APs purified from bovine brains determined that they are composed of two ~100 kDa large adaptin subunits (ß1 and γ for AP1, ß2 and α for AP2), one ~50 kDa medium subunit (µ1 for AP1 and µ2 for AP2) which has two folded domains connected by a linker and one ~18 kDa small subunit (σ1 for AP1 and σ2 for AP2).10 Malfunction of clathrin-dependent trafficking pathways have been associated with a variety of human diseases, such as, Alzheimer′s disease, schizophrenia, leukemias and X-linked mental retardation. Additionally, various pathogens take advantage of clathrin-mediated endocytosis to enter cells and use clathrin machinery to promote other aspects of infection.11

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

General description

Clathrin-mediated endocytosis is a key process in vesicular trafficking that transports various cargo molecules from the plasma membrane of eukaryotic cells into the cytoplasm. These cargoes are involved in various physiological processes, such as cell signaling and developmental regulation.8 Besides clathrin, coated vesicle populations contain the adaptor complexes Assemble Protein 1 (AP1) and Assemble Protein 2 (AP-2), also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor respectively.9 The adaptor proteins are major initiators and regulators of clathrin-coated vesicle trafficking that mediate the recruitment of clathrin to the membranes.8,10

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Specificity

Monoclonal Anti- γ-Adaptin antibody specifically recognizes 104 kDa polypeptide1 of the Golgi adaptor complex AP-1 from human, bovine, and monkey6. The antibody reacts with polypeptides of approximately 100 kDa in bovine liver,human heart fibroblasts, and Madin-Darby bovinekidney cultured cells (MDBK).1 The antibody does not recognize the γ-subunit in rodents.1 adaptor complex AP-1

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.